Broccoli Extract

Broccoli Extract

Main Active Ingredients:
Glucoraphanin — CAS: 21414-41-5 | Assay: 10%–20% (HPLC) | Broccoli Seed & Sprout
Sulforaphane— CAS: 4478-93-7| Assay: 5%–10% (HPLC) |
Myrosinase— CAS:9025-38-1| Assay: >200u/g |Red cabbage source
Application: dietary supplements | liver-support formulations | antioxidant & cellular-defense blends | skin-health supplements | cosmetic actives | nutraceutical & pharmaceutical ingredient applications
MOQ: 1KG
Certifications: Non-GMO, Kosher, Halal, FDA-Registered Facility, Third-Party COA

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Bulk Broccoli Extract Supplier — Glucoraphanin 10%/13%/20% HPLC, Sulforaphane 5%/10%, and Myrosinase Enzyme, B2B Wholesale

Written by Jonathan, Valeherb · Reviewed by Dr. Xiaoyan Qiu · Updated October 2026

Valeherb supplies three complementary broccoli extract ingredient lines for B2B formulators: glucoraphanin-standardized extract (Brassica oleracea var. italica, seed) at 10%, 13%, and 20% by HPLC; β-cyclodextrin-encapsulated sulforaphane at 5% (ambient-stable) and 10% (cold-chain); and myrosinase enzyme isolated from red cabbage (Brassica oleracea var. capitata rubra) at ≥220 U/g activity. Glucoraphanin is the dominant glucosinolate in broccoli seeds and the dietary precursor to sulforaphane: myrosinase cleaves glucoraphanin upon cell disruption or via gut bacterial activity, generating sulforaphane, which has been characterised in research as an activator of the Nrf2/ARE pathway and an inducer of phase II detoxification enzymes.[1] Valeherb provides HPLC-verified glucoraphanin content per batch for all glucoraphanin grades; sulforaphane content is confirmed by HPLC with β-cyclodextrin complex stability data on request; myrosinase activity is measured by in-house colorimetric assay (BST-QASOP261-00, sinigrin substrate).

Active Compounds

NOTE
The bioactivity summarised in this section is drawn from published research on the compounds and is provided for informational purposes only. It is not a health or efficacy claim for any finished product. Any structure/function claim on a finished good must be self-substantiated by the marketer and carry the required FDA disclaimer; disease treatment or prevention claims require drug approval.

Broccoli extract bioactivity is defined by glucoraphanin and its conversion product sulforaphane, acting through the Nrf2/ARE transcription pathway and phase II enzyme induction.[1] Glucoraphanin is the primary glucosinolate and HPLC standardization marker — chemically stable, biologically inactive until enzymatically converted. Sulforaphane binds Keap1 and releases Nrf2, which activates the ARE — upregulating cytoprotective genes including glutathione S-transferases (GSTs), NQO1, HO-1, and thioredoxin reductase. Myrosinase is the conversion enzyme; its presence and activity directly determine conversion efficiency — the full glucoraphanin vs sulforaphane conversion mechanism is covered in our companion guide. Indole glucosinolates (glucobrassicin) are co-present minor compounds studied for independent immune-modulating activity.

Compound Role in Formulation Processing Sensitivity
Glucoraphanin (glucosinolate) Primary HPLC standardization marker; stable sulforaphane precursor; Nrf2 pathway activator (via enzymatic conversion); antioxidant and detoxification-enzyme research relevance; broccoli seed extract identity marker Low — chemically stable; retained across standard extraction and drying; the stable form preferred for B2B ingredient supply
Myrosinase (enzyme) Conversion enzyme; cleaves glucoraphanin to produce sulforaphane; myrosinase-active grades provide immediate conversion potential; activity measured in units/gram by sinigrin-substrate colorimetric assay High — heat-labile above 60°C; inactivated by standard high-temperature spray drying; requires low-temperature processing and refrigerated storage to maintain activity
Sulforaphane (ITC bioactive) The active isothiocyanate; Nrf2/Keap1 pathway activator and phase II enzyme inducer; studied in research for antioxidant activity, skin cytoprotection, and inflammatory-pathway activity in preclinical models; β-cyclodextrin encapsulation used to stabilize pre-formed sulforaphane for supplement applications High in free form — unstable; rapidly degrades under heat, oxygen, and moisture. β-Cyclodextrin complex significantly improves ambient stability at 5%; 10% grade requires storage at −4°C to maintain potency
Indole glucosinolates (glucobrassicin) Minor co-compound; converts to indole-3-carbinol (I3C) in vivo; studied for immunomodulatory activity; present in whole-plant extracts Low — stable; present in whole-plant extracts alongside glucoraphanin
SOURCING NOTE
Three ingredient strategies are available for broccoli-based formulations. Glucoraphanin extract (stable precursor, 10–20% HPLC): shelf-stable, relies on in vivo myrosinase or gut bacterial conversion; the standard B2B bulk ingredient. Sulforaphane (β-cyclodextrin complex): delivers pre-formed active compound; 5% grade is ambient-stable, 10% grade requires cold storage at −4°C; preferred when formulators need a defined sulforaphane dose without co-supplying myrosinase. Myrosinase enzyme (red cabbage-derived, ≥220 U/g): supplied separately to co-formulate with glucoraphanin extract, enabling controlled in situ sulforaphane generation at the point of use. Each strategy has distinct stability, cold-chain, and regulatory implications — confirm which specification your formulation requires before sampling.

Available Grades and Specifications

All grades are verified per batch by the methods indicated. Certificates of Analysis available on request.

Glucoraphanin Extract (Broccoli Seed)

Grade Glucoraphanin Myrosinase Source Typical Application
Standard Seed Extract 10% HPLC Inactivated Broccoli seed Standard glucoraphanin capsules; Nrf2-focused formulations; liver-support supplements; most commonly specified commercial grade
High-Potency Seed Extract 13% HPLC Inactivated Broccoli seed Higher-specification Nrf2 formulations; premium cellular-defense supplements; cost-effective bridge between 10% and 20% grades
Ultra-Potency Seed Extract 20% HPLC Inactivated Broccoli seed Maximum glucoraphanin density per serving; high-specification research-grade formulations; low-volume, high-potency capsule formats

Sulforaphane — β-Cyclodextrin Complex

STORAGE
Free-form sulforaphane is highly unstable. Valeherb’s sulforaphane grades use β-cyclodextrin encapsulation to improve ambient stability. The 5% grade is suitable for ambient storage (cool, dry); the 10% grade requires storage at −4°C to maintain potency. Confirm cold-chain capability before ordering the 10% grade. Sulforaphane content is verified by HPLC; stability data available on request.
Grade Sulforaphane Encapsulation Storage Typical Application
Sulforaphane 5% (Ambient-Stable) 5% HPLC β-Cyclodextrin complex Ambient (cool, dry); recommend ≤25°C Supplement formulations requiring a defined sulforaphane dose without cold-chain dependency; blends, sachets, softgels
Sulforaphane 10% (Cold-Chain) 10% HPLC β-Cyclodextrin complex −4°C required High-potency sulforaphane supplements; research applications; formulators with cold-chain capability seeking maximum sulforaphane concentration per gram

Myrosinase Enzyme — Red Cabbage Source

Parameter Specification Method
Botanical source Red cabbage (Brassica oleracea var. capitata rubra) —
Thioglucosidase activity ≥220 U/g (batch result: 220.1 U/g) In-house colorimetric assay, sinigrin substrate (BST-QASOP261-00, Valeherb)
Appearance Light purple powder Visual inspection
Storage Ambient (cool, dry); activity declines above 60°C — avoid heat exposure —
Intended use Co-formulation with glucoraphanin extract to enable controlled in situ sulforaphane generation; not for standalone supplementation —
FORMULATOR NOTE
When co-formulating glucoraphanin extract with myrosinase enzyme, the ratio of enzyme activity (U/g) to glucoraphanin content determines conversion efficiency. Red cabbage-derived myrosinase is functionally equivalent to broccoli myrosinase for glucoraphanin hydrolysis; red cabbage sourcing provides a stable, scalable enzyme supply independent of broccoli seed availability. Contact Valeherb for co-formulation ratio guidance based on your target per-serving sulforaphane yield.

Formulation Applications

Application categories describe how downstream manufacturers use the ingredient. The research referenced below characterises the compounds, not any finished product.

Liver-Support & Detoxification Formulations

  • Broccoli extract standardized at 10–13% glucoraphanin in liver-support capsules formulated around the Nrf2 / phase II enzyme pathway (glutathione S-transferase, NQO1), characterised in published research[1]
  • Combination formulas pairing glucoraphanin extract with added myrosinase enzyme (from red cabbage) to maximize in situ sulforaphane conversion without cold-chain dependency
  • Combination formulas pairing this ingredient with NAC or milk thistle for complementary detoxification-pathway coverage

Antioxidant & Cellular-Defense Formulations

  • High-potency 20% glucoraphanin extract in cellular-defense and longevity-positioned capsules — Nrf2/ARE activation produces sustained phase II enzyme induction, a mechanism distinct from direct radical-scavenging antioxidants
  • β-Cyclodextrin sulforaphane (5% ambient-stable grade) in ready-to-mix powder formulations where a defined, immediate sulforaphane dose is required without relying on in vivo conversion
  • Sulforaphane has been studied in published research for phase II enzyme induction and cytoprotective pathway activation in laboratory models[1]; this is ingredient-level research and does not support disease claims on finished products

Skin-Health & Cosmetic Formulations

  • Beauty-from-within supplements where standardized broccoli extract is used for antioxidant and cellular-defense positioning; sulforaphane has been studied for HO-1 and thioredoxin reductase upregulation in skin-cell models
  • Topical cosmetic formulations — confirm INCI declaration (Brassica Oleracea Italica Seed Extract) and stability testing under formulation pH conditions

Research Background

Phase II enzyme induction was characterised in Zhang et al. (1992, Proceedings of the National Academy of Sciences), which isolated sulforaphane from broccoli and identified it as a potent inducer of phase II detoxification enzymes — quinone reductase and glutathione S-transferase — in laboratory models, establishing the mechanistic basis for Nrf2 / phase II cytoprotective pathway research.[1]

A human trial is reported in Kikuchi et al. (2015, World Journal of Gastroenterology): daily supplementation with glucoraphanin-rich broccoli sprout extract was associated with improvements in serum liver-enzyme markers (ALT and γ-GTP) in male subjects with elevated values, over an 8-week period.[2] For guidance on translating glucoraphanin content into a target intake, see how much sulforaphane per day research suggests.

Myrosinase’s role in sulforaphane bioavailability is characterized in Fahey et al. (2015, PLoS ONE): myrosinase status is a primary determinant of sulforaphane conversion — preparations relying on gut-microbial conversion show lower and more variable sulforaphane output than myrosinase-active formats.[3]

Sourcing Criteria

Parameter What to Verify Why It Matters
Glucoraphanin content (HPLC) Glucoraphanin % per batch; confirm 10%, 13%, or 20% grade matches per-serving dose requirement Primary potency marker; directly affects sulforaphane yield and Nrf2 activation per serving
Sulforaphane grade and storage Confirm HPLC %, β-cyclodextrin encapsulation, and storage requirement — 5% ambient or 10% at −4°C β-Cyclodextrin stability must match your supply-chain temperature capability; request stability data for target shelf life
Myrosinase status For glucoraphanin extract: inactivated (shelf-stable) or active. For enzyme grade: confirm activity U/g and assay method Determines in vivo conversion efficiency; U/g value enables co-formulation ratio calculation
Source and safety testing Broccoli seed (glucoraphanin grades); red cabbage (myrosinase). Heavy metals (ICP-MS), pesticide multi-residue screen per batch Seed material provides 10–100× higher glucoraphanin than florets; Brassica crops require batch-level pesticide screening

Regulatory Guidance

  • United States: Glucoraphanin extract is a recognized supplement ingredient; β-cyclodextrin is GRAS (GRN 000046). Structure/function claims require self-substantiation and FDA disclaimer; disease claims require drug approval.
  • European Union: Recognized food ingredient; no novel food assessment required. β-Cyclodextrin authorized under EU 1333/2008 (E 459). No authorized EFSA health claim for sulforaphane or glucoraphanin — Nrf2 or mechanism claims cannot be used on-pack without EFSA authorization.
  • China: Brassica oleracea extract recognized under GB standards. Export documentation available.

References

[1] Zhang Y, Talalay P, Cho CG, Posner GH. (1992). A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proceedings of the National Academy of Sciences. 1992;89(6):2399-2403. doi: 10.1073/pnas.89.6.2399

[2] Kikuchi M, Ushida Y, Shiozawa H, et al. (2015). Sulforaphane-rich broccoli sprout extract improves hepatic abnormalities in male subjects. World Journal of Gastroenterology. 2015;21(43):12457-12467. doi: 10.3748/wjg.v21.i43.12457

[3] Fahey JW, Holtzclaw WD, Wehage SL, Wade KL, Stephenson KK, Talalay P. (2015). Sulforaphane bioavailability from glucoraphanin-rich broccoli: control by active endogenous myrosinase. PLoS ONE. 2015;10(11):e0140963. doi: 10.1371/journal.pone.0140963

Reviewed byDr. Xiaoyan QiuBotanical Extract R&D Engineer · Associate Professor, Huaihua University. Research focus: medicinal plant resources, botanical extraction processes, and microbial fermentation technology — directly relevant to glucosinolate extraction and myrosinase-active extract processing.
Written byJonathanContent Editor, Valeherb. Specializing in botanical ingredient science communication for B2B nutraceutical and functional food markets.

Disclaimer: This article is intended for informational purposes only. All references describe published peer-reviewed research on the compounds and are not intended as medical advice or as claims for any product. These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Finished products must comply with applicable regulations in the buyer’s jurisdiction. © 2026 Valeherb. All rights reserved.

FAQ

A1:These are two distinct B2B ingredient strategies with important differences in stability, bioavailability, and formulation approach. Glucoraphanin is a stable glucosinolate that serves as the dietary precursor to sulforaphane — it is chemically stable in extract form and can be standardized by HPLC to a specific % per gram. Sulforaphane itself is an isothiocyanate that degrades rapidly when exposed to heat, oxygen, or moisture, making it extremely difficult to stabilize in supplement form at meaningful concentrations. Most commercially successful sulforaphane supplements are actually glucoraphanin-based, relying on enzymatic conversion in the gut. The conversion pathway requires myrosinase — either supplied with the extract (myrosinase-active format) or provided by gut bacteria (myrosinase-inactivated format). For formulators, glucoraphanin extract standardized for glucoraphanin % is the preferred B2B ingredient specification; “sulforaphane content” claims in stable extracts should be verified with stability data, as pre-formed sulforaphane in a stored extract is implausible without specialized stabilization technology.
A2:The difference is in vitro and in vivo sulforaphane conversion efficiency, with significant formulation implications. Myrosinase-active extract contains the enzyme alongside glucoraphanin — it begins converting to sulforaphane immediately when exposed to moisture, so it requires refrigerated storage (typically 2–8°C) and careful handling to prevent premature conversion during storage and manufacturing. When consumed, it provides efficient enzymatic conversion in the upper gut with predictable sulforaphane yield. Myrosinase-inactivated extract has the enzyme heat-denatured during processing — it is shelf-stable at room temperature for standard bulk shipping and storage, making it far more practical for typical supplement manufacturing. However, it relies on gut microbiome bacteria (primarily Bacteroides thetaiotaomicron and Lactobacillus species) for conversion, which produces approximately 20–30% lower and more variable plasma sulforaphane versus enzyme-active preparations. For maximum in vivo sulforaphane delivery, myrosinase-active extract is the superior specification. For ambient-temperature supply chain practicality, myrosinase-inactivated plus a myrosinase co-supplement (dried myrosinase from daikon or mustard seed) is a common formulation approach.
A3: It refers to whether the conversion enzyme is still functional in the extract. Myrosinase-active grades retain or re-add the enzyme, so they convert glucoraphanin to sulforaphane efficiently and reproducibly — but the enzyme is heat-sensitive (denatured above ~60°C), so these grades need low-temperature processing and refrigerated storage. Myrosinase-inactivated grades are shelf-stable and easier to handle but depend on gut-bacterial conversion, which is lower and varies between individuals. For B2B sourcing, confirm three things per batch: HPLC glucoraphanin %, myrosinase status (with activity units if active), and the resulting storage requirement.
A4:Minimum batch-level documentation: glucoraphanin % by HPLC per batch — the primary standardization marker; myrosinase status declaration — active (with enzyme activity units/gram) or inactivated, with storage requirements for active grades; source material declaration (broccoli seed, sprout, or floret) with extraction method; stability data confirming glucoraphanin retention under specified storage conditions; ICP-MS heavy metal panel (Pb, As, Cd, Hg) within USP/EU pharmacopeial limits; pesticide residue screen per EU 396/2005 or USP — Brassica crops require thorough pesticide screening; microbial limits. If the supplier claims measurable pre-formed sulforaphane content in a stable extract, request accelerated stability data confirming sulforaphane retention over shelf life — pre-formed sulforaphane in a non-stabilized extract is chemically implausible at meaningful concentrations. For EU market applications, request documentation confirming the product’s status under EU food supplement regulations and any EFSA claim substantiation available.

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