Broccoli Extract

Broccoli Extract

Main Active Ingredients:
Glucoraphanin — CAS: 21414-41-5 | Assay: 0.5%–20% (HPLC) | Broccoli Seed & Sprout
Application: dietary supplements | liver-support formulations | antioxidant & cellular-defense blends | skin-health supplements | cosmetic actives | nutraceutical & pharmaceutical ingredient applications
MOQ: 1KG
Certifications: Non-GMO, Kosher, Halal, FDA-Registered Facility, Third-Party COA

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Bulk Broccoli Extract Supplier — Glucoraphanin Standardized 0.5%–20% HPLC, with/without Myrosinase, B2B Wholesale

Written by Jonathan, Valeherb · Reviewed by Dr. Xiaoyan Qiu · Updated June 2026

Valeherb supplies broccoli extract (Brassica oleracea var. italica, seed and sprout) bulk wholesale, standardized for glucoraphanin content — 0.5%, 1%, 5%, 10%, and 13–20% glucoraphanin by HPLC — in myrosinase-active and myrosinase-inactivated (stable) formats, B2B. Glucoraphanin is the dominant glucosinolate in broccoli seeds and young sprouts and the dietary precursor to sulforaphane: myrosinase cleaves glucoraphanin upon cell disruption or via gut bacterial activity, generating sulforaphane, which has been characterised in research as an activator of the Nrf2/ARE pathway and an inducer of phase II detoxification enzymes.[1] The critical procurement distinction for broccoli extract is myrosinase status: myrosinase-active grades convert efficiently at room temperature but require refrigerated storage; myrosinase-inactivated grades are shelf-stable but rely on gut-microbial conversion, which research describes as producing lower and more variable sulforaphane yields.[3] Valeherb provides HPLC-verified glucoraphanin content per batch.

Active Compounds

NOTE
The bioactivity summarised in this section is drawn from published research on the compounds and is provided for informational purposes only. It is not a health or efficacy claim for any finished product. Any structure/function claim on a finished good must be self-substantiated by the marketer and carry the required FDA disclaimer; disease treatment or prevention claims require drug approval.

Broccoli extract bioactivity is defined by glucoraphanin and its conversion product sulforaphane, acting through the Nrf2/ARE transcription pathway and phase II enzyme induction.[1] Glucoraphanin is the primary glucosinolate and HPLC standardization marker — chemically stable, biologically inactive until enzymatically converted. Sulforaphane binds Keap1 and releases Nrf2, which activates the ARE — upregulating cytoprotective genes including glutathione S-transferases (GSTs), NQO1, HO-1, and thioredoxin reductase. Myrosinase is the conversion enzyme; its presence and activity directly determine conversion efficiency — the full glucoraphanin vs sulforaphane conversion mechanism is covered in our companion guide. Indole glucosinolates (glucobrassicin) are co-present minor compounds studied for independent immune-modulating activity.

Compound Role in Formulation Processing Sensitivity
Glucoraphanin (glucosinolate) Primary HPLC standardization marker; stable sulforaphane precursor; Nrf2 pathway activator (via enzymatic conversion); antioxidant and detoxification-enzyme research relevance; broccoli seed extract identity marker Low — chemically stable; retained across standard extraction and drying; the stable form preferred for B2B ingredient supply
Myrosinase (enzyme) Conversion enzyme; cleaves glucoraphanin to produce sulforaphane; myrosinase-active grades provide immediate conversion potential; activity measured in units/gram High — heat-labile above 60°C; inactivated by standard high-temperature spray drying; requires low-temperature processing and refrigerated storage to maintain activity
Sulforaphane (ITC bioactive) The active isothiocyanate; Nrf2/Keap1 pathway activator and phase II enzyme inducer; studied in research for antioxidant activity, skin cytoprotection, and inflammatory-pathway activity in preclinical models High — unstable; rapidly degrades in heat, oxygen, moisture; not a viable direct supplement ingredient; must be generated in situ from glucoraphanin by myrosinase
Indole glucosinolates (glucobrassicin) Minor co-compound; converts to indole-3-carbinol (I3C) in vivo; studied for immunomodulatory activity; present in whole-plant extracts Low — stable; present in whole-plant extracts alongside glucoraphanin
SOURCING NOTE
Three specifications define broccoli extract procurement. First, myrosinase status: active format requires cold chain but delivers efficient conversion; inactivated format is shelf-stable but relies on gut bacteria, giving lower and more variable sulforaphane yield — specify clearly. Second, standardization level: glucoraphanin % ranges from 0.5% (standard grade) to 20% (high-potency seed grade) — confirm which % your formulation requires based on per-serving dose calculations. Third, source material: broccoli seeds contain 10–100× higher glucoraphanin than mature florets; seed-derived material delivers higher potency per gram. If a supplier claims significant pre-formed sulforaphane content in a stable extract, request stability data — pre-formed sulforaphane degrades rapidly and is not plausible in standard stored extracts.

Available Grades and Specifications

All broccoli extract grades are HPLC-verified for glucoraphanin content per batch.

Grade Glucoraphanin Myrosinase Source Typical Application
Standard Broccoli Extract 0.5% HPLC Inactivated Sprout / floret Broad-spectrum greens supplement blends; entry-level glucoraphanin positioning; antioxidant formulas
Mid-Potency Extract 1–5% HPLC Inactivated Seed / sprout Liver-support supplements; Nrf2-focused formulations; standard glucoraphanin capsules
High-Potency Seed Extract 10–20% HPLC Inactivated Broccoli seed Premium cellular-defense supplements; high-specification Nrf2 formulations; highest glucoraphanin density per serving
Myrosinase-Active Extract 1–5% HPLC Active (units/g) Seed / sprout Maximum in vivo sulforaphane conversion; research applications; cold chain-maintained formulations

Formulation Applications

Application categories describe how downstream manufacturers use the ingredient. The research referenced below characterises the compounds, not any finished product.

Liver-Support & Detoxification Formulations

  • Broccoli extract standardized at 5–13% glucoraphanin in liver-support capsules formulated around the Nrf2 / phase II enzyme pathway (glutathione S-transferase, NQO1), characterised in published research[1]
  • Combination formulas pairing this ingredient with NAC or milk thistle for complementary detoxification-pathway coverage

Antioxidant & Cellular-Defense Formulations

  • High-potency 10–20% broccoli extract in cellular-defense and longevity-positioned capsules — Nrf2/ARE activation produces sustained phase II enzyme induction, a mechanism distinct from direct radical-scavenging antioxidants
  • Sulforaphane has been studied in published research for phase II enzyme induction and cytoprotective pathway activation in laboratory models[1]; this is ingredient-level research and does not support disease claims on finished products, which require separate regulatory authorization
  • Preclinical research has also examined glucoraphanin and sulforaphane in metabolic and adipose-tissue models

Skin-Health & Cosmetic Formulations

  • Beauty-from-within supplements where standardized broccoli extract is used for antioxidant and cellular-defense positioning; sulforaphane has been studied for HO-1 and thioredoxin reductase upregulation in skin-cell models
  • Topical cosmetic formulations — confirm INCI declaration (Brassica Oleracea Italica Seed Extract) and stability testing under formulation pH conditions

Research Background

Phase II enzyme induction was characterised in Zhang et al. (1992, Proceedings of the National Academy of Sciences), which isolated sulforaphane from broccoli and identified it as a potent inducer of phase II detoxification enzymes — quinone reductase and glutathione S-transferase — in laboratory models, establishing the mechanistic basis for Nrf2 / phase II cytoprotective pathway research.[1]

A human trial is reported in Kikuchi et al. (2015, World Journal of Gastroenterology): daily supplementation with glucoraphanin-rich broccoli sprout extract was associated with improvements in serum liver-enzyme markers (ALT and γ-GTP) in male subjects with elevated values, over an 8-week period.[2] For guidance on translating glucoraphanin content into a target intake, see how much sulforaphane per day research suggests.

Myrosinase’s role in sulforaphane bioavailability is characterized in Fahey et al. (2015, PLoS ONE): myrosinase status is a primary determinant of sulforaphane conversion — preparations relying on gut-microbial conversion show lower and more variable sulforaphane output than myrosinase-active formats.[3]

Sourcing Criteria

Parameter What to Verify Why It Matters
Glucoraphanin content (HPLC) Glucoraphanin % per batch by HPLC; confirm standardization level (0.5% through 20%) Primary potency marker; batch variability directly affects per-serving sulforaphane yield and Nrf2 activation
Myrosinase status Active (with activity units/gram) or inactivated; storage requirements if active Determines in vivo sulforaphane conversion efficiency; active = higher yield but cold chain required; inactivated = stable but lower, more variable yield from gut bacteria
Source material Broccoli seed, sprout, or floret; extraction method Seed delivers 10–100× higher glucoraphanin than florets; source determines potency ceiling and cost
Sulforaphane content (if claimed) Sulforaphane % if pre-converted extract is supplied Pre-formed sulforaphane degrades rapidly — if a supplier claims significant sulforaphane content in a stable extract, request stability data
Heavy metals (ICP-MS) Pb, As, Cd, Hg within USP <232> or EU pharmacopeial limits per batch Standard botanical extract requirement
Pesticide residues Multi-residue screen per EU 396/2005 or USP <561> per batch Brassica crops are a higher-risk category for pesticide residues; batch-level testing required
Stability data Glucoraphanin stability over shelf life under specified storage conditions Glucoraphanin is stable, but myrosinase-active extracts require refrigeration; stability data confirms storage-spec compliance

Regulatory Guidance

  • United States: Glucoraphanin-standardized broccoli extract is a recognized food/supplement ingredient. Any structure/function claim on the finished product must be self-substantiated by the marketer and carry the required FDA disclaimer; antioxidant-type claims are generally the most readily supported. Disease treatment or prevention claims are not permitted without drug approval or, for a specific qualified health claim, FDA review. Consult regulatory counsel. Organic grades require USDA NOP certification
  • European Union: Authorized food ingredient; glucoraphanin-standardized broccoli preparations are recognized under EU food regulations and do not require novel food assessment. There is currently no authorized EU health claim for sulforaphane or glucoraphanin — specific Nrf2, antioxidant, or mechanism claim language cannot be used on-pack without EFSA authorization. EU Organic (EC 834/2007) required for organic label claims
  • China: Brassica oleracea extract is recognized under GB standards as a food ingredient. Export documentation available. Organic grades include COFCC certification where required

References

[1] Zhang Y, Talalay P, Cho CG, Posner GH. (1992). A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proceedings of the National Academy of Sciences. 1992;89(6):2399-2403. doi: 10.1073/pnas.89.6.2399

[2] Kikuchi M, Ushida Y, Shiozawa H, et al. (2015). Sulforaphane-rich broccoli sprout extract improves hepatic abnormalities in male subjects. World Journal of Gastroenterology. 2015;21(43):12457-12467. doi: 10.3748/wjg.v21.i43.12457

[3] Fahey JW, Holtzclaw WD, Wehage SL, Wade KL, Stephenson KK, Talalay P. (2015). Sulforaphane bioavailability from glucoraphanin-rich broccoli: control by active endogenous myrosinase. PLoS ONE. 2015;10(11):e0140963. doi: 10.1371/journal.pone.0140963

Reviewed byDr. Xiaoyan QiuBotanical Extract R&D Engineer · Associate Professor, Huaihua University. Research focus: medicinal plant resources, botanical extraction processes, and microbial fermentation technology — directly relevant to glucosinolate extraction and myrosinase-active extract processing.
Written byJonathanContent Editor, Valeherb. Specializing in botanical ingredient science communication for B2B nutraceutical and functional food markets.

Disclaimer: This article is intended for informational purposes only. All references describe published peer-reviewed research on the compounds and are not intended as medical advice or as claims for any product. These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Finished products must comply with applicable regulations in the buyer’s jurisdiction. © 2026 Valeherb. All rights reserved.

FAQ

A1:These are two distinct B2B ingredient strategies with important differences in stability, bioavailability, and formulation approach. Glucoraphanin is a stable glucosinolate that serves as the dietary precursor to sulforaphane — it is chemically stable in extract form and can be standardized by HPLC to a specific % per gram. Sulforaphane itself is an isothiocyanate that degrades rapidly when exposed to heat, oxygen, or moisture, making it extremely difficult to stabilize in supplement form at meaningful concentrations. Most commercially successful sulforaphane supplements are actually glucoraphanin-based, relying on enzymatic conversion in the gut. The conversion pathway requires myrosinase — either supplied with the extract (myrosinase-active format) or provided by gut bacteria (myrosinase-inactivated format). For formulators, glucoraphanin extract standardized for glucoraphanin % is the preferred B2B ingredient specification; “sulforaphane content” claims in stable extracts should be verified with stability data, as pre-formed sulforaphane in a stored extract is implausible without specialized stabilization technology.
A2:The difference is in vitro and in vivo sulforaphane conversion efficiency, with significant formulation implications. Myrosinase-active extract contains the enzyme alongside glucoraphanin — it begins converting to sulforaphane immediately when exposed to moisture, so it requires refrigerated storage (typically 2–8°C) and careful handling to prevent premature conversion during storage and manufacturing. When consumed, it provides efficient enzymatic conversion in the upper gut with predictable sulforaphane yield. Myrosinase-inactivated extract has the enzyme heat-denatured during processing — it is shelf-stable at room temperature for standard bulk shipping and storage, making it far more practical for typical supplement manufacturing. However, it relies on gut microbiome bacteria (primarily Bacteroides thetaiotaomicron and Lactobacillus species) for conversion, which produces approximately 20–30% lower and more variable plasma sulforaphane versus enzyme-active preparations. For maximum in vivo sulforaphane delivery, myrosinase-active extract is the superior specification. For ambient-temperature supply chain practicality, myrosinase-inactivated plus a myrosinase co-supplement (dried myrosinase from daikon or mustard seed) is a common formulation approach.
A3: It refers to whether the conversion enzyme is still functional in the extract. Myrosinase-active grades retain or re-add the enzyme, so they convert glucoraphanin to sulforaphane efficiently and reproducibly — but the enzyme is heat-sensitive (denatured above ~60°C), so these grades need low-temperature processing and refrigerated storage. Myrosinase-inactivated grades are shelf-stable and easier to handle but depend on gut-bacterial conversion, which is lower and varies between individuals. For B2B sourcing, confirm three things per batch: HPLC glucoraphanin %, myrosinase status (with activity units if active), and the resulting storage requirement.
A4:Minimum batch-level documentation: glucoraphanin % by HPLC per batch — the primary standardization marker; myrosinase status declaration — active (with enzyme activity units/gram) or inactivated, with storage requirements for active grades; source material declaration (broccoli seed, sprout, or floret) with extraction method; stability data confirming glucoraphanin retention under specified storage conditions; ICP-MS heavy metal panel (Pb, As, Cd, Hg) within USP/EU pharmacopeial limits; pesticide residue screen per EU 396/2005 or USP — Brassica crops require thorough pesticide screening; microbial limits. If the supplier claims measurable pre-formed sulforaphane content in a stable extract, request accelerated stability data confirming sulforaphane retention over shelf life — pre-formed sulforaphane in a non-stabilized extract is chemically implausible at meaningful concentrations. For EU market applications, request documentation confirming the product’s status under EU food supplement regulations and any EFSA claim substantiation available.

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